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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Nat Methods. 2011 Nov 6;8(12):1041–1043. doi: 10.1038/nmeth.1770

Figure 1. Development of targeted proteomics assays using enriched in vitro synthesized full length proteins.

Figure 1

(a) Proteins for which targeted assays were built. (b) Schematic of the synthesis, enrichment, digestion and analysis of proteins to identify proteotypic peptides and their fragmentation patterns. (c) Protein samples were highly-enriched and full-length, as detected by silver-staining and immunodetection with an anti-schistosomal GST antibody. (d) SRM chromatographic traces from the NFIA peptide EDFVLTVTGK were readily detected over background. (e) Proteotypic peptides for EWSR1 were identified by comparing the signal intensity of all of the tryptic peptides monitored.