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. Author manuscript; available in PMC: 2012 Dec 7.
Published in final edited form as: J Am Chem Soc. 2011 Nov 11;133(48):19358–19361. doi: 10.1021/ja209391n

Figure 2.

Figure 2

Saturation kinetics of light, 15N-labeled and heavy HIV-1 protease. Steady state kinetic rates of product formation are plotted as a function of fluorescent substrate concentration. Catalysts are 10 nM light (blue), 15N (pink), and heavy (black) HIV-1 protease. Curves were plotted as a function of initial rate (vi) of product formation and substrate concentration and were fit to the equation vi = kcat[E][A]/(Km + [A]).