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. Author manuscript; available in PMC: 2013 Mar 1.

Published in final edited form as: Biochim Biophys Acta. 2011 Aug 5;1821(3):456–463. doi: 10.1016/j.bbalip.2011.07.020

Table 3.

Thermodynamic parameters for denaturation of apoA-I variants monitored by tryptophan fluorescence

apoA-I variant	GdnHCl-denaturation			urea-denaturation
apoA-I variant	ΔG°_D (kcal/mol)	m (kcal/mol)M⁻¹	D_½ (M)	ΔG°_D (kcal/mol)	m (kcal/mol)M⁻¹	D_½ (M)
WT	5.0 ± 0.1	4.4 ± 0.1	1.14 ± 0.05	5.9 ± 0.2	2.2 ± 0.1	2.7 ± 0.2
F225L/F229A/Y236A	5.4 ± 0.3	4.6 ± 0.2	1.18 ± 0.07	6.1 ± 0.2	2.2 ± 0.1	2.8 ± 0.1
F225L/F229L/A232L/Y236L	2.9 ± 0.1	2.5 ± 0.1	1.16 ± 0.03	4.8 ± 0.2	1.7 ± 0.1	2.8 ± 0.1