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Overexpression of wild-type PLD1 in NRK cells disrupts PLD1 perinuclear localization. Cells were transiently transfected with DNA encoding N-terminally HA-tagged PLD1 and stained with P1–P4 PLD1 antibodies (A and D) and monoclonal anti-HA antibodies (B and E). Top row, low level of HA-PLD1 expression; bottom row, high level of HA-PLD1 expression. Bar, 10 μm.
