Fig. 1.

S.so-HSP20 mRNA expression in S. solfataricus P2 analyzed by real-time PCR. The level of S.so-HSP20 mRNA was measured in cells cultured at the indicated growth temperature or cold shock at 4°C for 2 h, and normalized against NusG (Internal control) levels. The mRNA expression value at 75°C was set to 1 and the other values were normalized against this. Data are representative of three trials and were expressed as the mean±SEM. Statistical significance was performed using the two-tailed paired Student's t test. (**p < 0.01)