Table 2.

Strains and plasmids used in this work

Strain or plasmid	Relevant genotype or features	Reference
Strains
Sulfolobus solfataricus p2	Sulfolobus solfataricus DSMZ1617	DSMZ
E.coli BL21(DE3)	E.coli B (DE3)[F−dcm ompT hsdSB (r−Bm−B)]	Studier et al. 1990
E.coli P	E.coli BL21(DE3) with pET-28a	This work
E.coli H	E.coli BL21(DE3) with pET-28a-HSP20	This work
E.coli HR	E.coli DH5α with pUCm-T-HSP-RT	This work
E.coli NusG	E.coli DH5α with pUCm-T-NusG-RT	This work
Plasmids
pET-28a-HSP20	pET28a(+) with HSP20*	This work
pUCm-T-HSP-RT	pUCm-T with HSP-RT DNA	This work
pUCm-T-NusG	pUCm-T with NusG-RT DNA	This work

*HSP20, NCBI reference sequence: NP_343781.1 (She et al. 2001)