Table 2.

Comparison of disaggregation efficiencies between Tid1 splice variants

	J-protein	DnaK	Mortalin
ATPase (min−1)	Tid1-L	1.33 ± 0.085	1.99 ± 0.16
	Tid1-S	2.06 ± 0.057	1.93 ± 0.14
G6PDH disaggregation (min−1)	Tid1-L	7.29·10−5 ± 9·10−6	1.56·10−5
	Tid1-S	9.3·10−5	3.12·10−5
nmol ATP/nmol G6PDH refolded	Tid1-L	18,244	127,564
	Tid1-S	22,150	61,858

Turnover numbers of steady-state ATPase and G6PDH disaggregation were determined as described under “Materials and methods”; 16 μM of the indicated Hsp70 proteins, 1 μM of the relevant Tid1 construct, and 1 μM of Mge1 were used