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. Author manuscript; available in PMC: 2012 Nov 23.
Published in final edited form as: Cell. 2011 Nov 23;147(5):1066–1079. doi: 10.1016/j.cell.2011.10.039

Figure 1. Lin28B regulates let-7 biogenesis through a TUTase-independent mechanism.

Figure 1

(A) Schematic representation of human Lin28A and Lin28B (B) Western Blot analysis of Zcchc11, Lin28A, and Lin28B in extracts prepared from human cancer cell lines. (C) Co-immunoprecipitation (co-IP): Hela cells were co-transfected with Human myc-Lin28A, myc-Lin28B, or myc-Ago2 with either Flag-Zcchc11 or Flag-EIF6. Flag-IP and Flag- and Myc-western blots were performed to detect expression and interaction respectively. See Also Figure S1. (D) Stable knockdown of Zcchc11 leads to upregulation of mature let-7g levels in Lin28A-expressing cells but not Lin28B-expressing cell lines. miRNA levels were measured by q.RT-PCR. Error bars represent SEM (n=3). Protein knockdown was monitored by Western blot.