Figure 8.

Deletion of IRE1 prevents Kar2p secretion from an erv25Δ strain. (A) Amount of extracellular Kar2p in cultures from FY834 (WT), CBY114 (erv25 Δ), CBY427 (ire1 Δ), and CBY425 (erv25 Δ ire1 Δ) after 1 and 3 h. Proteins contained in the cell culture supernatant were concentrated by TCA precipitation and Kar2p was detected by immunoblot. (B) Intracellular levels of Kar2p, Gdi1p, and Erv25p from equal volumes of cell cultures grown as in A. (C) The same samples of precipitated proteins as in A were resolved on 12.5% SDS-PAGE and silver stained.