Table 1.
Quantitation of MT dynamic behavior in rhodamine tubulin-injected interphase cells, and in EGFP-tubulin-expressing cells in interphase and mitosis
| Dynamic Parameters | Interphase
cells
|
Mitotic cells
|
|
|---|---|---|---|
| Rhodamine tubulin 42 MTs | EGFP tubulin 62 MTs | EGFP tubulin 28 MTs | |
| Growth | |||
| Rate (μm/min) | 11.50 ± 7.14 | 11.50 ± 7.40 | 12.76 ± 5.66 |
| Distance (μm) | 1.10 ± 0.77 | 1.22 ± 0.93 | 3.14 ± 1.59a |
| Duration (s) | 6.25 ± 3.69 | 7.20 ± 4.96 | 17.0 ± 12.2a |
| Shrink | |||
| Rate (μm/min) | 14.8 ± 8.54 | 13.1 ± 8.43 | 14.14 ± 7.86 |
| Distance (μm) | 1.37 ± 1.37 | 1.52 ± 1.77 | 3.70 ± 3.83a |
| Duration (s) | 5.49 ± 3.41a | 6.71 ± 4.28 | 13.22 ± 7.65a |
| Average pause duration (s) | 18.3 ± 25.7b | 25.5 ± 32.7 | 9.31 ± 5.08a |
| % Time per phase (growth/shrink/pause) | 16.7/12.8/70.5 | 15/11.5/73.5 | 50.5/38.1/11.4 |
| Rescue frequency (s−1) | 0.203 ± 0.092 | 0.175 ± 0.104 | 0.045 ± 0.111a |
| Catastrophe frequency (s−1) | 0.035 ± 0.024 | 0.026 ± 0.024 | 0.058 ± 0.045a |
| Dynamicity (μm/min) | 4.5 ± 2.8 | 4.0 ± 3.5 | 14.6 ± 11.3a |
EGFP, enhanced green fluorescent protein; MT, microtubule.
a
Significant from EGFP tubulin interphase cells at 99.9% confidence level.
b
Significant from EGFP tubulin interphase cells at 95% confidence level.
All statistics analyzed using a Student's t test.