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. 2001 Apr;12(4):981–995. doi: 10.1091/mbc.12.4.981

Figure 6.

Figure 6

Inhibition of ESV formation by nonhydrolyzable analogues of GTP and neomycin. (A) ESV formation in vitro was assayed in the presence of nonhydrolyzable GTP analogues, 200 μM GTPγS or 200 μM GMP-PNP plus cytosol and an ATP-regenerating system. (B) ESV formation in vitro was assayed in the presence of varying concentrations of BFA. (C) ESV formation in vitro was assayed in the presence of varying concentrations of neomycin. Donor membranes and cytosol were preincubated with neomycin for 15 min at 0°C. (D) Inhibition of ESV formation by neomycin is prevented by the addition of poly-l-aspartic acid (PAA). Neomycin (0.3 mM) and/or poly-l-aspartic acid (0.03 mM) were preincubated with membranes and cytosol as above.