Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Feb 28;16(4):512–522. doi: 10.1634/theoncologist.2010-0174

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

©AlphaMed Press

PMC Copyright notice

Figure 3. — Characterization of the human ES cell lines Epi-544 and VAESBJ. (A): H&E, pan-cytokeratin, and vimentin immunohistochemical staining of the original human sample from which Epi-544 was derived. (B): Panel (from top) depicting cell morphology, karyotype, growth in soft agar, migration, and invasion. (C): ES cells do not express INI-1 protein (Western blot, upper panel) or mRNA (RT-PCR; lower panel) in contrast to NHFs and the leiomyosarcoma cell line SKLMS1, which were used as controls. (D): Epi-544 and VAESBJ preserve ES characteristics in vivo. Immunohistochemical staining of tumor xenografts is depicted (inset represents an INI-1⁺ soft tissue sarcoma xenograft).

Abbreviations: H&E, hematoxylin and eosin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; INI-1, integrase interactor 1; EMA, epithelial membrane antigen; ES, epithelioid sarcoma; NHF, normal human fibroblast; USEF, unclassified sarcoma with epithelioid features.