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Processing of Gas1p and CPY in PtdEtn depleted cells. (A) Western blot analysis of Gas1p from wild-type (lane 1), psd1Δ (lane 2), psd2Δ (lane 3), and psd1Δ psd2Δ (lanes 4 and 5) cells grown on synthetic medium. Growth of psd1Δ psd2Δ was supplemented with Etn (lane 4) or Cho (lane 5). (B) CPY was immunoprecipitated at 0, 15, and 30 min from wild-type and psd1Δ psd2Δ cells during a pulse-chase experiment with cells grown on synthetic medium supplemented with Cho.
