Table 1.
Strains and plasmids
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| FY1679 | MATa/MATα ura3-52/ura3-52 TRP1/trp1Δ63LEU2/leu2 Δ1 HIS3/his3 Δ200 | Winston et al. (1995) |
| YRB1 (wt) | MATa his3 Δ200 leu2 Δ1 trp1 Δ63 ura3-52 | This work |
| YRB2 | As YRB1 except psd1 Δ∷KanMX4 | This work |
| YRB3 | As YRB1 except psd2 Δ∷KanMX4 | This work |
| YRB4 | As YRB1 except MATα cho1 Δ∷KanMX4 TRP1 | This work |
| YRB5 | As YRB1 except psd1 Δ∷KanMX4 psd2 Δ∷KanMX4 TRP1 | This work |
| MSS204 | MATa dpl1 Δ∷LEU2 TPS2-LacZ leu2-3,112 ura3-52 trp1 his4 rme1 | R.C. Dickson, University of Kentucky |
| YRB6 | MATα psd1 Δ∷KanMX4 psd2 Δ∷KanMX4 dpl1 Δ∷LEU2his3 leu2 ura3-52 | This work |
| YRB7 | psd1 Δ∷KanMX4 dpl1 Δ∷LEU2 his3/4 leu2 ura3-52 | This work |
| YRB8 | psd2 Δ∷KanMX4 dpl1 Δ∷LEU2 his3/4 leu2 ura3-52 | This work |
| KS106 | MATα eki1 Δ∷TRP1 cki1 Δ∷HIS3 leu2-3,112 ura3-1 trp1-1 his3-11,15 ade2-1 can1-100 | Kim et al. (1999) |
| YRB9 | eki1 Δ∷TRP1 cki1 Δ∷HIS3 dpl1 Δ∷LEU2 his3 leu2 trp1-1 ura3 | This work |
| YRB10 | MATa/MATα psd1 Δ∷KanMX4/PSD1 psd2 Δ∷KanMX4/PSD2 dpl1 Δ∷LEU2/DPL1 eki1 Δ∷TRP1/EKI1 cki1 Δ∷HIS3/CKI1 his3/his3 leu2/leu2 trp1-1/TRP1 ura3/ura3 | This work |
| YRB11 | MATa/MATα psd1 Δ∷KanMX4/PSD1psd2 Δ∷KanMX4/PSD2 cki1 Δ∷HIS3/CKI1 his3/his3leu2/leu2 trp1/TRP1 ura3/ura3 | This work |
| YRB12 | psd1 Δ∷KanMX4 cki1 Δ∷HIS3 his3 leu2 ura3 | This work |
| YRB13 | psd2 Δ∷KanMX4 cki1 Δ∷HIS3 his3 ura3 | This work |
| YRB14 | psd1 Δ∷KanMX4 eki1 Δ∷TRP1 his3 leu2 ura3 | This work |
| YRB15 | psd2 Δ∷KanMX4 eki1 Δ∷TRP1 his3 leu2 ura3 | This work |
| YRB16 | psd1 Δ∷KanMX4 psd2 Δ∷KanMX4 eki1 Δ∷TRP1 his3 leu2 ura3 | This work |
| YRB17 | psd1 Δ∷KanMX4 eki1 Δ∷TRP1 cki1 Δ∷HIS3 his3 leu2 trp1-1 ura3 | This work |
| YRB18 | psd2 Δ∷KanMX4 eki1 Δ∷TRP1 cki1 Δ∷HIS3 his3 leu2 ura3 | This work |
| YRB19 | psd1 Δ∷KanMX4 cki1 Δ∷HIS3 dpl1 Δ∷LEU2 his3 leu2 trp1-1 ura3 | This work |
| YRB20 | psd2 Δ∷KanMX4 cki1 Δ∷HIS3 dpl1 Δ∷LEU2 his3 leu2 ura3 | This work |
| YRB21 | psd1 Δ∷KanMX4 eki1 Δ∷TRP1 dpl1 Δ∷LEU2 his3 leu2 trp1-1 ura3 | This work |
| YRB22 | psd2 Δ∷KanMX4 eki1 Δ∷TRP1 dpl1 Δ∷LEU2 his3 leu2 ura3 | This work |
| YRB23 | psd1 Δ∷KanMX4 psd2 Δ∷KanMX4 eki1 Δ∷TRP1 dpl1 Δ∷LEU2 his3 leu2 ura3 | This work |
| YRB24 | psd1 Δ∷KanMX4 eki1 Δ∷TRP1 cki1 Δ∷HIS3 dpl1 Δ∷LEU2 his3 leu2 trp1-1 ura3 | This work |
| YRB25 | psd2 Δ∷KanMX4 eki1 Δ∷TRP1 cki1 Δ∷HIS3 dpl1 Δ∷LEU2 his3 leu2 ura3 | This work |
| p24-3 | 3.2 kb PvuII-HindIII fragment with DPL1 cloned into YEp351 cut with SmaI and HindIII | R.C. Dickson, University of Kentucky |
| pRB1 | PSD1 ORF + about 3.6 kb 5′ and 2.2 kb 3′ flanking regions (about 7 kb insert) in YCp50 | This work |
| pRB2 | PSD2 ORF + about 8.1 kb 5′ and 7.3 kb 3′ flanking regions (about 19 kb insert) in YEp24 | This work |
| pRB3 | 2.6 kb SalI-NsiI fragment with PSD1 cloned into YEp352 cut with SalI and PstI | This work |