Table 3.
Carbon source dependent auxotrophies of strains with defects in PtdEtn biosynthesis grown on defined solid media
| Strain | Auxotrophy on glucose | Auxotrophy on lactate/ethanol |
|---|---|---|
| Wild type | None | None |
| psd1Δ | None | Etn or Cho or Ser |
| psd2Δ | None | None |
| psd1Δpsd2Δ | Etn or Cho | Etn |
| cho1Δ | Etn or Cho | Etn |
| psd1Δ psd2Δ dpl1Δ | Etn | Etn |
| psd1Δ psd2Δ+ 2μ DPL1 | None | None |
| cho1Δ+ 2μ DPL1 | None | None |
| psd1Δ dpl1Δ | None | Etn or Cho or Ser |
| psd2Δ dpl1Δ | None | None |
| psd1Δ eki1Δ | None | Etn or Cho or Ser |
| psd1Δ cki1Δ | None | Etn |
| psd1Δ cki1Δ eki1Δ | None | Lethala |
| psd2Δ eki1Δ | None | None |
| psd2Δ cki1Δ | None | None |
| psd2Δ cki1Δ eki1Δ | None | None |
| psd1Δ cki1Δ dpl1Δ | None | Etn |
| psd1Δ eki1Δ dpl1Δ | None | Etn or Cho or Ser |
| psd2Δ cki1Δ dpl1Δ | None | None |
| psd2Δ eki1Δ dpl1Δ | None | None |
| psd1Δ psd2Δ cki1Δ | Lethal | Lethal |
| psd1Δ psd2Δ cki1Δ eki1Δ | Lethal | Lethal |
| psd1Δ psd2Δ cki1Δ dpl1Δ | Lethal | Lethal |
| psd1Δ cki1Δ eki1Δ dpl1Δ | None | Lethala |
| psd2Δ cki1Δ eki1Δ dpl1Δ | None | None |
| psd1Δ psd2Δ cki1Δ eki1Δ dpl1Δ | Lethal | Lethal |
| psd1Δ psd2Δ eki1Δ | Etn or Cho | Etn |
| psd1Δ psd2Δ eki1Δ dpl1Δ | Etn | Etn |
| cki1Δ eki1Δ dpl1Δ | None | None |
a
poor growth on full medium