Figure 2. Involvement of GRKs in GR internalization.
HEK-GR cells transiently transfected with GRK2, GRK3, GRK5 or empty vector plasmids (control) were serum-starved for 1 h and treated with 100 nM glucagon for 30 min. Receptor internalization was assessed by binding (Method B). Data represent the mean ± s.e.m. of 4 experiments. **; p < 0.01 vs. control; ***, p<0.001 vs. control.