. Author manuscript; available in PMC: 2012 Nov 29.

Published in final edited form as: Biochemistry. 2011 Nov 8;50(47):10318–10327. doi: 10.1021/bi201279u

Table 1.

Summary of DEER-derived distance distributions for IPSL-537 and IPSL-401.

DEER Sample	x₁	r₁	w₁	x₂	r₂	w₂
537/401 apo, (−) H₂O₂	0.781	2.13	1.72	0.219	4.02	2.03
537/401 apo, (+)H₂O₂	0.815	2.20	1.20	0.186	2.96	2.30
537/401 actin-bound, (−)H₂O₂	0.396	1.84	2.01	0.604	3.81	1.21
537/401 actin-bound, (+)H₂O₂	0.545	2.34	1.58	0.455	3.77	1.04

Simulated spectra were fit to experimental spectra as described in Experimental Procedures. Fitting parameters for i=1, 2, or 3 Gaussian distance distributions are defined as follows: r_i is the center distance in nm, w_i is the full width half-maximum in nm, and x_i is the mole fraction. Uncertainties were estimated from SD of repeated experiments (n = 3–5). For distances 1.6 to 3.0 nm, fractional uncertainties in r and w were approximately 12%.