Table 3.
Treatment (group) | Days after Rx | Apomorphine revolutions before Rx | Apomorphine revolutions after Rx | Striatal TH (pmol/h/mgp) |
---|---|---|---|---|
Saline | 3 | 324 ± 69* | 401 ± 72 | 162 ± 36 |
6 | 232 ± 43 | 342 ± 62 | 288 ± 42 | |
10 | 228 ± 23 | 331 ± 55 | 120 ± 18 | |
| ||||
Clone 877 | 3 | 196 ± 24 | 56 ± 20b | 4286 ± 918b |
6 | 231 ± 47 | 230 ± 55 | 1015 ± 213a | |
10 | 219 ± 35 | 256 ± 27 | 335 ± 79a | |
| ||||
prgTH2 | 3 | 251 ± 38 | 123 ± 31b | 1343 ± 176b,c |
6 | 295 ± 55 | 119 ± 34a | 1535 ± 324b | |
10 | 344 ± 27 | 443 ± 70c | 884 ± 7b,d | |
| ||||
prgTH3 | 3 | 317 ± 23 | 234 ± 38d | 167 ± 35d |
6 | 197 ± 11 | 127 ± 11a | 545 ± 117a | |
10 | 202 ± 31 | 111 ± 9b,d | 985 ± 101b,d | |
| ||||
prgTH4 | 3 | 211 ± 28 | 100 ± 9b | 1219 ± 137b,c |
6 | 234 ± 37 | 113 ± 20b | 1621 ± 196b | |
10 | 3 ± 64 | 249 ± 64 | 500 ± 86b | |
| ||||
None | NA | 0 | 0 | 5068 ± 168 |
Rx: single IV injection of TfRMAb-targeted THLs encapsulating the respective TH expression plasmid. *Mean ± S.E. (n = 3–6 rats per point). Statistically significant differences were determined by Student's t-test.
Clone 877 is the TH expression plasmid driven by the SV40 promoter. For details in the engineering of TH genomic vectors, clones prgTH2–TH4, please see Figure 5.
Difference with saline: P < 0.05a; P < 0.005b.
Difference with clone 877: P < 0.05c; P < 0.005d.
NA: not applicable.
From [48].