Figure 1.

Hepatocytes and testes from rats fed a Se-deficient diet for 12 wk have the same level of PHGPx mRNA as the corresponding tissues from rats fed a Se-supplemented diet. Rat pairs and hepatocyte RNAs were identical to those described in Moriarty et al. (1998). (A) Total RNA (25 μg) was electorphoresed in agarose, transferred to a nylon membrane, and hybridized to ³²P-labeled cDNAs for rat PHGPx (a gift from Donna Driscoll; Pushpa-Rekha et al., 1995), β-actin (Moriarty et al., 1998), and GPx1 (Moriarty et al., 1998). The levels of PHGPx and GPx1 mRNAs were normalized to the level of β-actin mRNA and presented as a percentage of the normalized level in Se-fed animals, which was defined as 100%. N.D., not detectable. Results are shown for one pair of animals. (B) RT-PCR analysis of PHGPx and β-actin mRNAs was as described (Moriarty et al., 1997, 1998), except that PHGPx mRNA was analyzing using primers described in MATERIALS AND METHODS. The left-most four lanes assay a serial dilution of testis RNA and demonstrate that the analysis is quantitative.