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pPHGPx harboring either the TGA Sec codon or one of several nonsense codons generates mRNA that is subject to NMD in NIH3T3 and H35 cells. NIH3T3 and H35 cells were treated and analyzed as described in the legend to Figure 3 except that pPHGPx plasmids were used in the place of pmCMV-PHGPx plasmids. Values did nor vary by >5–7% in two independently performed experiments.
