Figure 7.

Se deficiency also augments the NMD of endogenous PHGPx mRNA in untransfected H35 cells. Untransfected H35 cells were cultured as described in the legend to Figure 6 except that insulin and transferrin were replaced by 10% FBS where specified and the concentration of sodium selenite was as specified. For each lane, the level of endogenous PHGPx mRNA was normalized to the level of endogenous TPI mRNA, and normalized levels were expressed relative to the level in the absence of sodium selenite (SeO₃), which was defined as one for cells cultured in insulin + transferrin as well as cells cultured in the presence serum.