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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Gene Ther. 2010 May 13;17(10):1200–1205. doi: 10.1038/gt.2010.66

Figure 2. In vitro analysis of SK-N-AS/MMP9 cells.

Figure 2

Untransfected and MMP-9 transfected SK-N-AS cells were plated in Matrigel-coated or uncoated (control) transwell chambers in triplicate. After 22h, cells were scraped from the upper surface of the membrane and cells attached to the lower surface were photographed (A) and counted (B). C. SK-N-AS and SK-N-AS/MMP9 spheroid cultures were infected with 5×104 pfu of JD0G virus and GFP fluorescence visualized at 24hpi in whole-mount spheroids (top) or by two-photon microscopy of fixed spheroids (bottom). Scale bars, 200μm. D. Individual spheroids (n=5) were trypsinized and GFP-positive cells counted under a fluorescent microscope.