Figure 3.
ATP and NEM effect on EMTCs formation. (a) Commassie blue R-250 staining of a 5–20% SDS-PAGE showing that in the absence of ATP or myc-NSF, or in the presence of 1 mM NEM-modified NSF, EMTCs do not form. The only protein bands detected are the light (Lc) and the heavy (Hc) chains of the anti-myc antibody and myc-NSF, when present. When 0.5 mM ATP and myc-NSF are present EMTCs are assembled in both cytosol (C) and Triton X-100 extract of membrane fraction (M). (b) Comparison between the protein composition of EMTCs (C) and a protein pattern obtained from a whole HMECs cytosolic preparation (C1) on a one-dimensional 5–20% SDS-PAGE.