Figure 6.

Rab 5 is a protein component of EMTCs. GTP overlay reveals the presence of GTP binding proteins along gradient fractions derived from both cytosol (C) and Triton X-100 extract of the membrane preparation (M). Rab 5 (25 kDa) has been identified by coimmunoprecipitation and immunoblotting experiments as a protein component of EMTCs. Rab 5 seems to be more concentrated in membrane than in cytosol gradient fractions. The highest concentration was found at the bottom of the membrane gradient.