Table 1.
Balb/c – GFP P. yoelii 17X and 17XL murine malaria model.
| Day p.i. | 3 | 4 | ||||||
|---|---|---|---|---|---|---|---|---|
| Line | L-GFP | L | NL-GFP | NL | L-GFP | L | NL-GFP | NL |
| Parasitaemia (%) | 1.1 ± 0.3 | 1.1 ± 0.2 | 1.1 ± 0.2 | 1.1 ± 0.2 | 9.9 ± 1.4 | 10.3 ± 0.4 | 9.2 ± 0.6 | 9.8 ± 0.2 |
| Reticulocytemia (%) | 2.7 ± 0.4 | 2.9 ± 0.7 | 2.5 ± 0.4 | 2.8 ± 0.5 | 2.3 ± 0.3 | 2.4 ± 0.5 | 2.9 ± 0.5 | 2.7 ± 0.3 |
| pRet/pRBCs (%) | 60.0 ± 9.1 | 62.8 ± 4.6 | 69.2 ± 9.5 | 72.7 ± 5.8 | 23.4 ± 5.1 | 24.7 ± 4.0 | 32.2 ± 10.0 | 38.0 ± 14.6 |
| ring/pRBCs (%) | 31.4 | 30.8 | 39.2 | 31.8 | ||||
| troph/pRBCs (%) | 64.7 | 62.7 | 57.3 | 63.5 | ||||
| sch/pRBCs (%) | 3.9 | 6.7 | 3.4 | 4.8 | ||||
| S. w. (g) | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.3 ± 0.0 | 2.2 ± 0.0 |
| RP/WP | 2.3 ± 0.3 | 2.0 ± 0.2 | 0.8 ± 0.2 | 0.8 ± 0.1 | ||||
Different parameters were calculated in Balb/c mice infected with wild-type and transgenic parasites on days 3 and 4 p.i. when all experiments were done. Parasitaemias were calculated in groups of 12 animals. Reticulocytemia and percentage of parasitized reticulocytes (pRet) were measured in at least six mice per group. Percentages of asexual forms [rings, trophozoites (troph) and schizonts (sch)] were assessed in four mice per group counting at least 250 pRBCs per animal. Spleen weight (S.w.) and ratio of red pulp to white pulp area (RP/WP) were measured in 3–4 animals per group. The results are expressed as mean ± SEM. Statistical analysis was used to compare differences between the lethal and non-lethal group on those days, with no significant differences found.