Fig. 3.

Expression of Npy1r mRNA and Y1R peptide in the brain of control and conditional mutants raised by FVB/J and C57BL/6J dams. “FVB/J” and “C57BL/6J” refer to foster mother strain. (A) Representative autoradiograms of in situ hybridization of Npy1r mRNA on brain coronal sections of Npy1r^2lox and Npy1r^rfb mice fostered to FVB/J (Left) and to C57BL/6J (Right) dams. (Scale bar: 1.5 mm.) (B Left) Quantitative signal intensity (OD) analysis of in situ hybridization revealed the strongest significant decrease of Npy1r mRNA expression in CA1 and DG cell bodies of FVB/J fostered Npy1r^rfb mice compared with their control littermates. (B Right) Quantitative signal intensity (OD) analysis of in situ hybridization revealed no significant differences between C57BL/6J fostered Npy1r^rfb and Npy1r^2lox mice. A decrease of Npy1r mRNA expression was detected in the CA1, CA3, and DG of C57BL/6J fostered Npy1r^2lox compared with FVB/J fostered Npy1r^2lox mice (Left). (A and B) Data are expressed as optical density and are the mean ± SEM from two independent experiments; n = 3–4. Two-way ANOVA revealed a significant effect of genotype for DG [F_(1,10) = 68.0; P < 0.001], a significant effect of genotype and foster mother strain for CA1 [F_(1,10) = 7.32; P < 0.05 and F_(1,10) = 8.37; P < 0.05, respectively] and a significant effect of foster mother strain for CA3 [F_(1,10) = 11.04; P < 0.01]. *P < 0.01 and ^#P < 0.05 versus Npy1r^2lox mice fostered to FVB/J dams, by Newman–Keuls. CA1, CA1 stratum pyramidale; CA3, CA3 stratum piramidale; DG, dentate gyrus; BLA, basolateral amygdala; CeA, central amygdala; MeA, medial amygdala. (C) The immunohistochemical anti-Y1R staining confirmed the reduced Y1R expression in cellular layers of DG and CA1 of FVB/J fostered Npy1r^rfb mice compared with their Npy1r^2lox littermates. (Scale bar: 50 μm.) The hypothalamic Y1R immunosignal was similar in the PVN [magnocellular (PVNm) and medial parvocellular (PVNmp)] division and in the arcuate (ARC) nuclei (Scale bar: 25 μm).