Fig. 3.

Effects of BIG1, BIG2, KANK1, or KIF21A depletion on wound healing. (A) Cells were lysed 48 h after transfection with nontargeted (NT), BIG1, BIG2, KANK1, or KIF21A siRNA or vehicle alone (Mock) and amounts of proteins quantified by densitometry of Western blots. (B) Images of cells at 0 and 6 h after wounding as in A. (Scale bar, 70 μm.) (C) Covered area is the difference between wound areas 0 and 6 h after wounding. Data are means ± SEM of values from five experiments. **P < 0.005 *; P < 0.05, (two-tailed t test) for difference from cells transfected with nontargeted siRNA. (D) Migration paths of five representative cells at wound edges during 6-h assays (B). Initiation of cell migration = 0. (E–G) Motility characteristics in B and C were calculated for individual cells as described in Materials and Methods. Data are means ± SEM of values from three experiments for at least 120–150 cells in each group. **P < 0.005; *P < 0.05, (ANOVA test) for difference from cells transfected with vehicle alone (Mock).