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. 2011 Oct 7;118(22):5832–5839. doi: 10.1182/blood-2011-05-353961

Figure 6.

Figure 6

Ionizing radiation induces Blimp-1 expression independently of TACI. (A-B) X-ray irradiation of 18.81 cells (A) or C57BL/6-TACI-ko cells (B) induces γH2AX foci, indicating accumulation of DNA double-strand breaks. Cytospin slides of nonirradiated (0 Gy) or irradiated (4 Gy) cells were stained with an anti-γH2AX antibody (top diagrams) and counterstained with 4,6-diamidino-2-phenylindole (bottom diagrams). (C) Irradiation of 18.81 B cells with 1 Gy or 4 Gy induces Blimp-1 expression transiently, detected by quantitative RT-PCR. Relative expression of Blimp-1 was calculated by determining the ratio Blimp-1/β-actin for each sample. Values reflect 3 independent measurements. (D) Irradiation induces Blimp-1 expression independently of TACI. TACI-ko B cells, isolated from C57BL/6-TACI-ko mice, were irradiated with 4 Gy and cultured with 15 μg/mL of LPS for 24 hours. Blimp-1 expression increased after irradiation of TACI-deficient B cells. RNA was obtained after 24 hours in culture and expression of Blimp-1 or β-actin analyzed by quantitative RT-PCR. Relative expression of Blimp-1 was calculated by determining the ratio Blimp-1/β-actin for each sample. Values reflect 3 independent measurements. Averages were compared by t test. Significant differences were noted: *P < .05, **P < .01, and ***P < .001.