Fig. 7.
Characterization of glioma xenograft with sGC transfectants. H.E. staining showed tumor mass derived from sGC α1β1Cys105-overexpressing cells with well defined pathological features of glioblastoma (A; 100× and 400× magnification). The xenografts with α1β1Cys105 sGC are composed of heterogeneous populations of tumor cells (A, 400× magnification) with different nuclear density. The tumor tissue derived from α1β1Cys105 sGC transfected clone had significantly fewer microvessels according to CD31 staining (B and C; 200× magnification). Significant reduction in Ki-67 staining, a marker associated with cell proliferation, was observed in sGC transfectant-derived tumors (D and E; 200× magnification). Data are mean ± S.E.M. n = 3 to 4 for each group. **, P < 0.01(versus control groups).