Figure 3.

YNBY does not have anti-bacterial capabilities against E. coli K12. 50 μL of E. coli K12 culture (OD=0.6) was incubated on plates with various concentrations of YNBY (B-F) overnight, at 37°C on LB agar plates. E. coli was also incubated with 50 μg/ml of antibiotic ampicillin (Amp) as a positive control (A), in which no bacteria grew (indicated by translucence). In contrast, bacterial lawns formed at all doses of YNBY (B-F). The hand-written indication at the top of each plate denotes the concentrations of the original YNBY stocks for our reference during the experiments. This suggests that YNBY is not anti-bacterial. At least five independent experiments were performed.