Figure 4.

YNBY exhibits dose-dependent cytotoxic-ity to both T- and B- lymphocytes, more potently than 6-mercaptopurine (6-MP) and 5-aminosalicylic acid (5-ASA), two of the most commonly used IBD medications. Total protein expression in cell lysates was used as an indicator of change in cell numbers, as analyzed bySDS-PAGE. A. As the concentration of YNBY treatment increased, the total proteins reflected on the gel decreased dramatically for both B-cells and T-cells (Molt4 and Jurkat), indicating a drastic decrease in cell numbers. B and C. Cytotoxicity of 6-MP (B) or 5 -ASA (C) to Molt4 cells was shown for comparison purpose with YNBY (A). These studies demonstrate that YNBY exhibited more cytoxicity to lymphocytes than 6-MP and 5-ASA. At least four independent experiments were performed.