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Localization of Ste6 by immunofluorescence. The c-myc-tagged Ste6 variant, encoded by pYKS2, was detected with anti-myc primary antibodies and FITC-conjugated anti-mouse secondary antibodies in different strains. (A) JD52 (wild type). (B) JD116 (doa4). (C) RKY975 (pep4). (D) RKY1449 (doa4 pep4). Left, FITC-fluorescence; right, Nomarsky image. The cells were grown at 30°C in minimal medium with 1% casamino acids.
