Figure 5.

Ste6 is sequestered from the cytoplasm in a pep4 mutant. The wild-type strain RKY1392 (lanes 1, 2, and5) and the congenic Δpep4 strain RKY1582 (lanes 3 and4), which both contain a CUP1p-STE6-HIS3 cassette integrated into their genome, were transformed with the vector plasmid YEplac181 (lanes 1 and 3) or the 2μ-VPS4 plasmid pRK567 (lanes 2 and 4). Lane 6, doa4 strain RKY1651 containing the CUP1-STE6-HIS3 cassette. An equal amount of cells was spotted onto minimal plates containing different supplements (required nutrients, +/−histidine, +/−0.5 mM CuSO₄), as indicated. Plates were incubated for 3 d at 30°C.