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. 2011 Nov 14;4:216. doi: 10.1186/1756-3305-4-216

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Copyright ©2011 Côrtes et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Scanning electron microscopy showing the interaction of promastigotes and Lulo cells. Electron micrographs show adhesion of parasites from three Leishmania species (P) with Lulo cells (L). A - Lulo cells; in vitro, present rounded morphology or sprawled with cytoplasmic projections. B - Leishmania (V.) braziliensis; adhesion occurs by flagellum (arrows) with tenuous contact between both cells and parasite. C-D - Interaction of species from the subgenus Leishmania with Lulo cells; occurs by both flagellum and body. C- Leishmania (L.) amazonensis; promastigotes are adhered by both body and flagellum (arrows) to cytoplasmic membrane of Lulo cells. D - Interaction of Lulo cells with Leishmania (L.) chagasi; occurs with participation of cytoplasmic projections involving the flagellum (arrows). In detail, we show flagellum (arrows) and body (arrows) of parasites that are being enveloped by several cytoplasmic projections of Lulo cells - inset - D. Images were assessed after 24 h (D) and 48 h (B and C) of interaction.