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. 2011 Nov 24;2011:636497. doi: 10.1155/2011/636497

Figure 1.

Figure 1

Comparative analysis of CAR promoter activity. (a) Schematic description of CAR gene promoter containing specific transcription factor binding sites and its deletion constructs. The luciferase reporter gene constructs were cloned into pGL3 vector. (b, c) Each CAR promoter-luciferase reporter construct was cotransfected into two different CAR-positive cells with β-galactosidase gene. *P = 0.0003, **P < 0.001 relative to other constructs, not pGL3. Bars represent SD. (d) Either Wt or mut of the core promoter (−585/−400) was cotransfected into four different CAR-positive cells with β-galactosidase gene. From total cell extracts, luciferase activities were examined and normalized by β-galactosidase activity. The relative luciferase activities were determined by statistical analysis of three independent experiments. #P < 0.05 relative to each of wild-type. Bars represent the mean ± SD.