Figure 3.

Sp1 binds to its putative binding motif within the core promoter. (a) Labeled probe and cold wild or mutant type probes used for competition assay were used as described in Section 2. The closed triangle indicates the Sp1-DNA probe complex. (b) Labeled probe was preincubated with either MTM or DMSO (vehicle) for 1 h at 4°C before incubation with the nuclear proteins. (c, d) Chromatin immunoprecipitation assay. Each of cell lysates was extracted from MCF-7 cells or HepG2 (e) cells after treatment either by TSA or MTM. Chromatin immunoprecipitation was performed with anti-Sp1, anti-acetyl histone H3, and then PCR was carried out with primers flanking Sp1 binding site.