Figure 6.

Abnormal actin organization in ain1 deletion and fim1 deletion cells. (A) Failure of actin-ring formation in ain1-Δ1 cells under stress conditions. Strains 972 (left panels) and JW45 (right panels) growing in YES medium at 30°C were shifted to EMM + 1 M KCl medium at 18°C for 20 h, fixed, and double-stained with rhodamine-phalloidin (upper panels) and bisBenzimide (lower panels). (B) Mislocalization and disorganization of actin patches in fim1-Δ1 cells. Strains 972 (left panels) and JW142 (right panels) growing in EMM medium at 25°C were shifted to 36°C for 5 h, fixed, and double-stained with rhodamine-phalloidin (upper panels) and bisBenzimide (lower panels). Bar, 10 μm.