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Cell division defect of an ain1 fim1 double mutant. ain1-Δ1 41nmt1-fim1+ cells (strain JW164) growing in EMM medium without thiamine (inducing conditions) at 25°C (A) or 16 h after a shift to YES medium (repressing conditions, leading to depletion of Fim1p) at 25°C (B–E) were double-stained for septa and DNA without fixation (A and B), stained for septa alone without fixation (C), or double-stained for F-actin (D) and DNA (E) after fixation (see MATERIALS AND METHODS). Bar, 10 μm.
