Table 3.
Genetic interactions of ain1-Δ1 with other mutations affecting medial-ring positioning and/or organization
| Mutant | Growth at the indicated
temperaturea
|
||
|---|---|---|---|
| 25°C | 30°C | 32°C | |
| ain1-Δ1 | ++++ | ++++ | ++++ |
| act1-48 | +++ | +++ | +++ |
| ain1-Δ1 act1-48 | − | + | + |
| myo2-E1 | +++ | +++ | +++ |
| ain1-Δ1 myo2-E1 | + | − | − |
| cdc3-6 | +++ | +++ | ++ |
| ain1-Δ1 cdc3-6 | + | − | − |
| rng2-D5 | ++++ | +++ | ++ |
| ain1-Δ1 rng2-D5 | +++ | ++ | − |
| mid1-ΔF | +++ | +++ | +++ |
| ain1-Δ1 mid1-ΔF | ++ | ++ | ++ |
Double mutants were constructed by crossing appropriate strains (Table 1), dissecting tetrads, and incubating at 25 or 32°C as needed to recover viable double-mutant clones. To evaluate growth at different temperatures, cells were diluted to yield single colonies on YES medium containing phloxin B (see MATERIALS AND METHODS) and incubated at the temperatures indicated. (++++) Colony formation similar to wild type and nearly all cells healthy (phloxin-pink); (+++) medium-size colonies with some dead cells (phloxin-red); (++) small colonies with substantial numbers of dead cells (phloxin-red); (+) very slow growth, tiny colonies with large numbers of dead cells (phloxin-dark red); (−) no colony formation.