Table 5.
Set of genome specific primer pairs used to produce TILLING 1th PCR amplicons.
| Amplicon | Oligo-forward (5'-3') | Oligo-reverse (5'-3') | T. annealing | Size (bp) |
|---|---|---|---|---|
| A(II-V) | cgctcgctcgctccaatc | gcaactggtcagtattcagtaagctaag | 65°C | 1720 |
| A(VI-IX) | tctgagaatatgctgggacgtag | gttcgaaaatgctacatgctca | 62°C | 1560 |
| A(X-XIII) | ccagtggtcagaatgcatcaac | gggaactatctaagactccgtagcac | 67°C | 2100 |
| B(IV-IX) | atgtggtggatgggttatgg | tccatagaataaaccatcagaccg | 62°C | 1970 |
| D(II-VI) | atcgcgcttcctgaacctg | gggctgaagcttaagacactgac | 65°C | 1980 |
| D(X-XIII) | gaggcagtgggcatgtgaaagtc | ctagggaactatctaagactccgtagcac | 67°C | 2200 |