Table 6.
Set of gene specific primer pairs used to produce TILLING 2nd PCR amplicons.
| Allele | Exon | primer forward (5'-3') | Primer Reverse (5'-3') | Size (bp) | 
|---|---|---|---|---|
| II | ccactgaccgactcact | atggacggggagattgg | 215 | |
| III | tcactattgtagtcatccttgcat | tgaagatttcccggcacg | 156 | |
| IV | tggtttcgttagtctgctct | tgagcgaaagtagcggg | 313 | |
| V | tttgggtatgcctccgt | tggaggcgcttcataatact | 163 | |
| VI | ttgctctaaatttatgatctggct | aggtggaagattgccaag | 153 | |
| SBEIIa-A | VII | tgctcctattgatgccgat | gctacatgctcaactaaataattgg | 152 | 
| VIII | ctctgcccactaagggt | aaatttcatttaataatgtaatggagatcg | 204 | |
| IX | ccttttgtgaccatttactaaggata | accagaaacaggtgaaataact | 157 | |
| X | acaatacttagaggatgcatctga | ggtgaagaggcgcataca | 212 | |
| XI | ggtatttctgacttgtatgaccatt | accagataaacagtaaagcagc | 223 | |
| XII | gttgcattgcttcatcaatgatt | caaatatggtgacagaagtcagag | 237 | |
| XIII | tgttaaatctgttcttacacatgtcg | catagcaattatttcagtgccct | 266 | |
| IV | aacacactgctaaatttgaatgat | agactagtggaggcgtt | 19 | |
| V | tgctgaaggtatcatctaattgc | tgaccattaacaatagattagaaggtg | 159 | |
| SBEIIa-B | VI | cagttactctaaatttatgatctggct | aggtggaagattgccaag | 134 | 
| VII | cctattgatgccgatatttgatatg | tcctcgactaaataattggccag | 152 | |
| VIII | aactctgcctactaagggt | acactggaaattccatttaataatgtaac | 204 | |
| IX | ccttttgtgaccatttactaaggata | ccggaaacaggtgaaataact | 157 | |
| II | actattgtagtcatccttgcatt | atgaagatttaccggcacg | 157 | |
| III | tcagtctgctctacaattgctat | gaaagcagcgggtaggc | 301 | |
| IV | gggtatacctcggtggattc | agactagtggaggcgttt | 167 | |
| V | gaaggtatcgtctaattgcatatct | caataaattggaaggtgtctcgtt | 154 | |
| SBEIIa-D | IX | accatttactaaggatatttacatgcaa | accagaaacaggtgaaataact | 151 | 
| X | acaatacttagaggatgcatctga | ggtgaagaggcgcataca | 212 | |
| XI | ggtatttctgacttgtatgaccatt | accagataaacagtaaagcagc | 223 | |
| XII | gttgcattgcttcatcaatgatt | caaatatggtgacagaagtcagag | 237 | |
| XIII | tgttaaatctgttcttacacatgtcg | catagcaattatttcagtgccct | 266 | |