Table 6.
Set of gene specific primer pairs used to produce TILLING 2nd PCR amplicons.
Allele | Exon | primer forward (5'-3') | Primer Reverse (5'-3') | Size (bp) |
---|---|---|---|---|
II | ccactgaccgactcact | atggacggggagattgg | 215 | |
III | tcactattgtagtcatccttgcat | tgaagatttcccggcacg | 156 | |
IV | tggtttcgttagtctgctct | tgagcgaaagtagcggg | 313 | |
V | tttgggtatgcctccgt | tggaggcgcttcataatact | 163 | |
VI | ttgctctaaatttatgatctggct | aggtggaagattgccaag | 153 | |
SBEIIa-A | VII | tgctcctattgatgccgat | gctacatgctcaactaaataattgg | 152 |
VIII | ctctgcccactaagggt | aaatttcatttaataatgtaatggagatcg | 204 | |
IX | ccttttgtgaccatttactaaggata | accagaaacaggtgaaataact | 157 | |
X | acaatacttagaggatgcatctga | ggtgaagaggcgcataca | 212 | |
XI | ggtatttctgacttgtatgaccatt | accagataaacagtaaagcagc | 223 | |
XII | gttgcattgcttcatcaatgatt | caaatatggtgacagaagtcagag | 237 | |
XIII | tgttaaatctgttcttacacatgtcg | catagcaattatttcagtgccct | 266 | |
IV | aacacactgctaaatttgaatgat | agactagtggaggcgtt | 19 | |
V | tgctgaaggtatcatctaattgc | tgaccattaacaatagattagaaggtg | 159 | |
SBEIIa-B | VI | cagttactctaaatttatgatctggct | aggtggaagattgccaag | 134 |
VII | cctattgatgccgatatttgatatg | tcctcgactaaataattggccag | 152 | |
VIII | aactctgcctactaagggt | acactggaaattccatttaataatgtaac | 204 | |
IX | ccttttgtgaccatttactaaggata | ccggaaacaggtgaaataact | 157 | |
II | actattgtagtcatccttgcatt | atgaagatttaccggcacg | 157 | |
III | tcagtctgctctacaattgctat | gaaagcagcgggtaggc | 301 | |
IV | gggtatacctcggtggattc | agactagtggaggcgttt | 167 | |
V | gaaggtatcgtctaattgcatatct | caataaattggaaggtgtctcgtt | 154 | |
SBEIIa-D | IX | accatttactaaggatatttacatgcaa | accagaaacaggtgaaataact | 151 |
X | acaatacttagaggatgcatctga | ggtgaagaggcgcataca | 212 | |
XI | ggtatttctgacttgtatgaccatt | accagataaacagtaaagcagc | 223 | |
XII | gttgcattgcttcatcaatgatt | caaatatggtgacagaagtcagag | 237 | |
XIII | tgttaaatctgttcttacacatgtcg | catagcaattatttcagtgccct | 266 |