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Intra and extra-cellular expression analysis of EPO with and without Kozak sequence addition, through ELISA. Optical density was measured at 450 nm. Both intra and extra-cellular EPO expression increased after the addition of Kozak sequence. (A) Comparison of extra-cellular EPO expression with Kozak (pcDNA3.1-K-SS-EPO) and without Kozak sequence (pcDNA3.1-SS-EPO) expression vectors. (B) Comparison of intra-cellular EPO expression after the addition of Kozak.
