Figure 3. Confirmation of siRNA silencing activity.

N2a and CLHA-02-ATRT cells at 50% confluence were transfected with 30 nM siRNA targeted against MLH1 and PMS2 and a negative control scrambled siRNA for a period of 5 hours. At 72 hours post-transfection cells were lysed and total RNA extracted. Reverse transcription PCR analyses were conducted and confirmed successful knockdown of MLH1 and PMS2 expression in both cell types following siRNA transfection. Beta actin expression is shown for both the control and targeted siRNA treated cells to confirm equal sample loading.