Figure 6. Spheroid-derived cells show an increased popensity to form new spheroids.

N2a and CHLA-02-ATRT MCTS were separated from 7 day old cultures using the method described previously in this paper. The MCTS and remaining non-spheroid-forming monolayer cells were treated with non-enzymatic cell dissociation solution to produce single cell suspensions and re-seeded as separate populations into new 60 mm dishes at a density of 1×10³ cells per dish. Spheroid-derived cells exhibited a higher propensity to form MCTS compared to monolayer-derived cells. Each bar represents the mean +/− s.e.m. for at least three independent experiments. Significant differences (P<0.05) are indicated by the asterisks (*) and were calculated using the Student's t-test.