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. 2011 Dec 1;6(12):e28355. doi: 10.1371/journal.pone.0028355

Figure 3. Adrenal glands of Prop1 deficient mice are not hypotrophic.

Figure 3

Adrenal glands were dissected from 5 and 8 week old female N4 B6 Prop1+/+ and Prop1-/- mice, fixed, embedded, sectioned, and stained with hemotoxylin and eosin (Panels A, C, E, G) and immunostained for 20α-hydroxysteroid dehydrogenase [59] and developed with diaminobenzidine (brown, Panels B, D, F, H) to visualize the X-zone (brackets). The ratio of adrenal weight to body weight (Panel I) was increased in Prop1 -/- (n = 5) compared to Prop1+/− (n = 6) or Prop1+/+ (n = 3) N4 B6 male mice at 8 to 10 wks. Values represent the mean adrenal weight (mg) per body weight (g) ± SE. *, P<0.0001; **, P<0.0005.