Figure 4.

Effect of benzothiazole based compounds on cell cycle regulatory proteins Cyclin D1, Skp2 in HepG2 cells. HepG2 cells treated at 80% confluency with YM, A3 and A4 at 4 μM concentration for 24 h. The total cell lysates were isolated and analysed by immunoblotting with anti-CyclinD1, anti-Skp2. YM is the positive control. β-actin was used as loading control. Western blots were representative of three independent experiments. C represents control untreated cells. The molecular weight of CyclinD1 and Skp2 were 35, 47 KDa. Each experiment was repeated three times and densitometric analysis was done. Results were statistically significant with p-value <0.001 for A3 and A4 when compared to control.