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Immunofluorescence based studies of PTEN after treatment with benzothiazole based compounds. HepG2 cells were grown in 10% FBS containing medium in coverslips and treated with 4 μM of effective benzothiazole compound (A4). Cells were fixed and processed for immunofluorescence study as described in material and methods. Images were captured in confocal microscopy. The pattern of staining was also recorded. Control: represents untreated cells.
