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. 2011 Dec 1;7(12):e1002392. doi: 10.1371/journal.ppat.1002392

Figure 5. Deletion of the TgKu80 gene in TATi transactivator parasite line.

Figure 5

(A) PCR analysis using primers specific for the TgKu80 coding region (left), targeting construct integration (middle), or an unrelated control region (right) using genomic DNA from RHΔHX, TATIΔTgKu80 and RHΔTgKu80 [38] as template. Position of primers is indicated in panel B. (B) Schematic representations of homologous recombination between the BLE modified TOXOW30 cosmid and the TgKu80 locus. The probe used for the southern blot presented in panel C is indicated and sizes expected after BglII digest are shown. (C) Southern blot analysis of the TATiΔTgKu80 clone and its parental TATi line demonstrating TgKu80 disruption.