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. 2011 Dec 1;7(12):e1002392. doi: 10.1371/journal.ppat.1002392

Table 1. Summary of the bioinformatics and experimental data for each of the eleven new apicoplast proteins found in this work.

Gene ID predicted product predicted SP/TMD predicted Domains homology Pf homologue PlasmoAP Sub localization Cosmid result of cosmid attempt
TGME49_110770 hypothetical protein, conserved SP, TMD* (TMHMM AA9–31) 0044270 Thioredoxin-like algae and apicomplexans PFE1450c no periphery PSBLW05 resistance, no KO
TGME49_021920 hypothetical protein none NifU NifU-like domain, NIFU NIFU NITROGEN-FIXING C-TERMINAL FIXATION NITROGEN NIFU-LIKE DOMAIN CLUSTER HESB/YADR/YFHF:NITROGEN-FIXING plants none - lumen PSBMB21 KO (not essential)
TGME49_021330 DNA gyrase subunit A, putative none 0035588 Type II DNA topoisomerase, DNA gyrase A, more cyanobacteria, algae PFL1120c yes lumen NA
TGME49_008840 ATP-dependent DNA helicase, putative none 0044179 Nucleic acid-binding proteins, 0044263 P-loop containing nucleoside triphosphate hydrolases, ultradead3, HELICASE_ATP_BIND_1 Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile, more none - lumen PSBM109 KO (not essential)
TGME49_087270 hypothetical protein TMD (TMHMM 87-109) none algae and apicomplexans PFL0600w yes periphery ToxoW30 resistance, no KO, essential
TGME49_039680 hypothetical protein, conserved none none apicomplexan unique/specific PFE1075c yes lumen PSBMG78, PSBLE72 resistance, no KO
TGME49_091670 RNA helicase, putative SP 0044263 P-loop containing nucleoside triphosphate hydrolases, ultradead3, DEAD DEAD/DEAH box helicase, HELICASE_ATP_BIND_1 Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile, more only Tg, plants and algae, few mammalians PF10_0209 no lumen NA
TGME49_059230 hypothetical protein SP, TMD* (TMHMM AA20-42) 0045587 lambda integrase-like, N-terminal domain, SAP SAP motif profile apicomplexan unique/specific MAL13P1.42 yes lumen NA
TGME49_039320 hypothetical protein, conserved TMD (TMHMM AA54-76) 0041621 BolA-like, BolA BolA-like protei red algae, plants no - lumen NA
TGME49_002440 hypothetical protein SP none only Tg, Nc, Phaeodactylum tricornutum no - in and near or around the apicoplast NA
TGME49_001270 hypothetical protein none none Only Tg, Nc, Theileria PF10_0246 yes periphery TOXOZ18, TOXPD77, TOXPI78 cosmid sequence rearranged