Table 1. Minicell production in C. jejuni ΔflhG upon exposure with cephalexin.
Cell Lengtha | ||||||
Strain | Time | Treatment | <0.5 µm (minicells) | 0.5–1 µm | 1–2 µm | >2 µm |
Wild-type | 0 h | none | 1.6±0.8 | 8.1±0.2 | 84.5±1.1 | 5.8±0.5 |
Wild-type | 6 h | none | 2.3±0.8 | 3.8±0.1 | 78.2±3.7 | 15.8±4.0 |
Wild-type | 6 h | cephalexinb | 1.5±1.5 | 3.9±1.4 | 62.9±13.6 | 31.8±10.7 |
ΔflhG | 0 h | none | 25.6±2.0 | 18.6±4.1 | 52.5±2.5 | 3.3±0.4 |
ΔflhG | 6 h | none | 31.3±2.0 | 6.3±0.3 | 57.2±4.5 | 5.2±2.2 |
ΔflhG | 6 h | cephalexinc | 13.8±1.1 | 4.0±0.9 | 44.8±10.1 | 37.4±9.9 |
The long axes of the cell bodies of individual bacteria were measured. The data are reported as percentage of the populations (± standard error) that had bodies between the indicated lengths.
The strain was grown with 15 µg/ml cephalexin for 6 hours before analysis.
The strain was grown with 12.5 µg/ml cephalexin for 6 hours before analysis.